Purification and Characterization of an l-Amino Amidase from Mycobacterium neoaurum ATCC 25795.

University of Groningen Purification and Characterization of an L-Amino Amidase from Mycobacterium neoaurum

Chemo-enzymatic synthesis of amino acids and derivatives

A short overview is given of new developments in the chemo-enzymatic synthesis of amino acids. Amino acid resolution procedures based on stereoselective enzymatic hydrolysis of amino acid amides have been developed. Two enzymes, an aminopeptidase from Pseudomonas putida and an amidase from Hycobacterium neoaurum have been purified to homogeneity. In addition, methods for the stereoselective syn...

Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism.

The catabolism of sterols in mycobacteria is highly important due to its close relevance in the pathogenesis of pathogenic strains and the biotechnological applications of nonpathogenic strains for steroid synthesis. However, some key metabolic steps remain unknown. In this study, the hsd4A gene from Mycobacterium neoaurum ATCC 25795 was investigated. The encoded protein, Hsd4A, was characteriz...

Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes

BACKGROUND The strategy of modifying the sterol catabolism pathway in mycobacteria has been adopted to produce steroidal pharmaceutical intermediates, such as 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), which is used to synthesize various steroids in the industry. However, the productivity is not desirable due to some inherent problems, including the unsatisfactory uptake rate and the low ...

Mycobacterium neoaurum and Mycobacterium bacteremicum sp. nov. as causes of mycobacteremia.

Reference isolates of Mycobacterium neoaurum, Mycobacterium aurum, and the nonvalidated species "Mycobacterium lacticola" were the focus of two recent molecular taxonomic studies. On the basis of this grouping, we identified 46 clinical pigmented, rapidly growing mycobacterial isolates. By 16S rRNA gene sequencing, only two major taxa were identified: M. neoaurum and a previously uncharacterize...